Authenticity Assessment of Fruit Juices using LC-MS/MS and Metabolomic Data Processing

نویسندگان

  • Lukas Vaclavik
  • Ondrej Lacina
  • André Schreiber
  • Jana Hajslova
چکیده

Liquid Chromatography coupled to tandem Mass Spectrometry (LC-MS/MS) with an AB SCIEX 4000 Q TRAP system was used for comprehensive fingerprinting of several fruit juices. Metabolomic data processing tools were used for authentication, i.e. classification of juices and adulteration detection. The developed statistical model was able to reliably detect 25% of orange juice adulteration with apple or grapefruit juice. In addition, high resolution and accurate mass MS and MS/MS measurements using the AB SCIEX TripleTOF 5600 system were performed to identify characteristic markers for fruit juice authenticity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of fruit juice authenticity using UPLC-QToF MS: a metabolomics approach.

In recent years, with the growing complexity of global food supply chains and trade, food fraud, including adulteration of high value foods with cheaper substitutes, has become an increasingly important issue. A metabolomics approach can be applied to discover biomarkers that can be used to trace food adulteration. A study was undertaken to discover novel, potential biomarkers for the rapid det...

متن کامل

QuEChERS Sample Preparation for LC/MS and LC/UV Determination of Carbendazim and Other Conazole Fungicides in Orange Juice

IN T RODUC T ION QuEChERS methodology is commonly used for sample preparation of fruits and fruit juices, prior to LC/MS or other analytical chromatography. Recently, a nonregistered fungicide residue, carbendazim, was detected in orange juice purchased at a local market. This juice was produced from orange juice concentrate imported into the USA. In this application note, we discuss sample pre...

متن کامل

Flavonoid composition of Citrus juices.

In the early nineties the presence of flavonoids in Citrus juices began to attract the attention of a number of researchers, as a result of their biological and physiological importance. This short review will explore two different aspects. The first part will focus on analytical techniques for the characterization of juices from different Citrus fruits regarding their flavonoid content (even i...

متن کامل

A Guideline to Univariate Statistical Analysis for LC/MS-Based Untargeted Metabolomics-Derived Data

Several metabolomic software programs provide methods for peak picking, retention time alignment and quantification of metabolite features in LC/MS-based metabolomics. Statistical analysis, however, is needed in order to discover those features significantly altered between samples. By comparing the retention time and MS/MS data of a model compound to that from the altered feature of interest i...

متن کامل

An R package to process LC/MS metabolomic data: MAIT(Metabolite Automatic Identification Toolkit)

Processing metabolomic liquid chromatography and mass spectrometry (LC/MS) data files is time consuming. Currently available R tools allow for only a limited number of processing steps and online tools are hard to use in a programmable fashion. This paper introduces the metabolite automatic identification toolkit MAIT package, which allows users to perform endto-end LC/MS metabolomic data analy...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2012